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Coding
Part:BBa_K3778027:Design
Designed by: Yun Tsai Group: iGEM21_CCU_Taiwan (2021-09-05)
Thrombin releasable TAT
Subparts|Ruler|SS|DSScars: Show|HideVertical: Show|HideLength: UnknownGet part sequenceView plasmid
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Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We modify the last two amino acids, glycine (G) and serine (S), in the thrombin cleavage site to fit the first two amino acids of TAT (BBa_K1202006) to avoid extra residues at the N-terminal of TAT after thrombin cleavage.
Source
TAT (Trans-Activator of Transcription) protein (BBa_K1202006) modified by CCU_Taiwan team.